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Vitamin A-E Serum HPLC Analysis Kit

Vitamin A-E Serum HPLC Analysis Kit

Zivak Vitamin A-E HPLC Analysis Kit was developed for rapid, sensitive and reliable quantitative detection of the Vitamin A-E concentrations in human serum samples and it gives results in 8 minutes with minimal sample preparation. Main methods and procedures that we selected are based on EN ISO 13485 and 98/79/EC.

The level of vitamin A in the plasma or serum is a reflection of the quantities of vitamin A and carotene ingested and absorbed by the intestine.

Vitamin A plays an essential role in the function of the retina (adaptation to dim light), is necessary for growth and differentiation of epithelial tissue, and is required for growth of bone, reproduction, and embryonic development. Together with certain carotenoids, vitamin A enhances immune function, reducing the consequences of some infectious diseases. Vitamin A in excess can be toxic.

Vitamin E contributes to the normal maintenance of biomembranes, the vascular system, and the nervous systems; and provides antioxidant protection for vitamin A. Deficiency of vitamin E in children leads to reversible motor and sensory neuropathies; this problem also has been suspected in adults.

Deficiencies of vitamins A and E may arise from poor nutrition or from intestinal malabsorption. People, especially children, at risk include those with bowel disease, pancreatic disease, chronic cholestasis, celiac disease, cystic fibrosis, and intestinal lymphangiectasia.

Vitamin A-E Serum HPLC Analysis Kit 0
HPLC-UV Parameters

Device

Zivak ASP-100 HPLC-UV System

Column

Zivak Vitamin A-E Serum HPLC Analytical Column  

Injection Volume

20 µL

Gradient

00.00 min                            100% A

08:00 min                            100% A

Flow

1.20  mL/min

Detector

UV/VIS

Column Oven

30 °C

Wavelength

00.00-02.00 min                            325nm

02:00-08,00 min                            295nm

 
 
Analytical Performance

No

Analyte

LOD (mg/L)

LOQ (mg/L)

Linearity (r2)

Accuracy %

Precision (%CV)

1

Vitamin A

0,02

0,06

0,999

95

3,7

2

Vitamin E

0,35

1,05

0,998

96

4,2

 

Storage & Stability:

This analysis kit can be shipped at room temperature.

Reagent 1, MP, WB should be stored at room temperature.

Calibrator and controls should be stored at 2-8 °C.

After arrival, reagent 2, Calibrator and controls (after reconstitution) must be stored at -20 °C.

All the other components of the kit should be stored at room temperature. Please make sure that you keep reagent 1 at room temperature otherwise it will become unusable.

All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

Preparation of Calibrators & Controls:

Add 5 ml deionized water to serum calibrator/control and dissolve.

After preparation, calibrators and controls must be aliquoted into 250 µl volumes and stored at -20 °C.

 

Sample Pretreatment:

1. Take 200 mL of patient sample (or calibrators and controls) into the sample preparation tube.

2. Add 250 mL Reagent 1 and vortex for 10 seconds.

3. Add 200 mL Reagent 2 and vortex for 10 seconds.

4. Centrifuge at 12000 x rpm for 3 minutes.

5. Take the upper phase into the autosampler vial.

6. Inject 20 mL to the HPLC system.

Note: The prepared sample is stable at 2-8°C for 24 hours.

Materials Supplied

Order No.

Volume

Component

ZV-4013-02R1-10

1x 50ml

Reagent 1, Contains aqueous buffer

ZV-4013-02R2-10

1x 40ml

Reagent 2, Contains deuterated internal standard

ZV-4013-02WB-10

1x 0,5 L

Washing Solution

ZV-4013-02MP-10

1x 2,5 L

Mobile Phase, Contains organic solvent

ZV-4013-KK-10

1 x 1 pc

User Guide

 
 
Materials Required But Not Supplied

Order No.

Volume

Component

ZV-4013-02S1-10

1 x 5 ml

Lyophilized Serum Calibrator Level 1

ZV-4013-02K1-10

1 x 5 ml

Lyophilized Serum Control Level 1

ZV-4013-02K2-10

1 x 5 ml

Lyophilized Serum Control Level 2

ZV-4013-02C1-10

1 pcs

Zivak Vitamin A-E Serum HPLC Analytical Column

 
File Name Type Size Download
User Manual 798.26 KB

Zivak General Catalog

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