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Metanephrines LC-MS/MS analysis kit

Metanephrines LC-MS/MS analysis kit
CE-IVD certified and suitable for any kind of LC-MS/MS instrument

 

The Zivak Metanephrines and Normetanephrine LC-MS/MS analysis kit can be used in combination with the Zivak-Multitasker as well as manually. Analysis run time for the manual version is just 13minutes. Due to the efficient and quite smart procedure of the Zivak-Multitasker, the automated version guarantees also to receive results in 13 minutes per test, including sample preparation-, sample injection- and analysis run time.

 

Quantitative LC-MS/MS analysis kit for Metanephrine and Normetanephrine in urine.

Metanephrine, Normetanephrine are primary metabolites of the epinephrine and norepinephrine catecholamines respectively. Urinary concentration of metanephrines is used to evaluate suspected pheochromocytoma. Despite the rarity of pheochromocytoma, the dangers of uncontrolled severe hypertension and the very effective surgical treatment of this condition mean that diagnosis is important. Urinary or plasma catecholamines or catecholamine-derivatives are commonly used to screen for pheochromocytomas prior to imaging. 

Zivak Urinary Metanephrines LC-MS/MS Analysis Kit was developed for accurate analysis of Metanephrine and Normetanephrine in urine samples. Main methods and procedures that we selected are based on EN ISO 13485 and 98/79/EC.

Metanephrines LC-MS/MS analysis kit 0
LC-MS/MS Parameters

Device

LC-MS/MS Analysis System

Column

Zivak Metanephrine Urine LC-MS/MS Analytical Column

Injection Volume

20 µL

Pump Program

00:00  min                     100 % A

01:30   min                    100 % A

03:30  min                     60   % A

07:00  min                     60   % A

08:00  min                     100 % A

13:00   min        100 % A

Flow

0.30 mL/min

Ionization Mode

ESI positive

CID Gas

2.4 mTorr

API Nebulizing Gas

50 psi

Scan Time

1.0 sec

SIM Width

1.5 amu

Drying Gas

35 psi

Drying Gas Temp.

350 °C

Needle

6000V

Shield

600V

Capillary

80V

Detector

1600 V

 
Analytical Performance

No

Analyte

LOD (µg/L)

LOQ

(µg/L)

Accuracy

(%)

Intra-Assay

Precision (%CV)

Inter-Assay

Precision

(%CV)

Linearity

(R2)

1

Metanephrine

2.6

7.8

98.3

1.7

2.5

0.9989

2

Normetanephrine

2.9

9.1

97.9

2.1

3.1

0.9990

3

3-methoxytyramine

1.1

3.4

98.2

3.8

4.6

0.9967

 
Storage Conditions:
  • This analysis kit can be shipped at room temperature.
  • Standards and controls, Reagent 1, 2, should be stored at 2-8 °C.
  • Standards and controls (after reconstitution) should be stored at - 20 °C.
  • All other components of the kit should be stored at between room temperature.
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

 

Preparation of Calibrators & Controls:

  1. Add 8 ml of 0.2 M HCl to urine calibrator or control and dissolve.
  2. After preparation, calibrators & controls must be aliquoted into 450 µl volumes and stored at -20 °C.

 

Sample Pretreatment (Manual):

  1. Take 400 mL of patient sample (or calibrators and controls) into a 15 mL glass tube.
  2. Add 100 mL of Reagent 1 and vortex for 10 seconds.
  3. Add 50 mL of Reagent 2 and vortex for 10 seconds.
  4. Incubate 90-100 °C for 40 minutes.
  5. Wait for 10 minutes and vortex for 10 seconds.
  6. Filter liquid with injector and 0.45 um filter.
  7. Take filtered sample into autosampler vial.
  8. Inject 20 mL to the LC-MS/MS system.

Note: The prepared sample is stable at 2-8°C for 24 hours.

Materials Supplied

Order No.

Volume

Component

ZV-3032-02R1-20

1x 20 ml

Reagent 1, Contains deuterated internal standards

ZV-3032-02R2-20

1x 10 ml

Reagent 2, Contains base

ZV-3032-02MA-20

1 x 1 L

Mobile Phase A, Contains organic solvent

ZV-3032-02MB-20

1 x 0,5 L

Mobile Phase B, Contains organic solvent

ZV-3032-02WB-10

 1 x 1 L

Washing Solution, Contains organic solvent

ZV-3032-KK-20

1x 1 pc

User Guide

 
 
Materials Required But Not Supplied

Order No.

Volume

Component

ZV-3032-02S1-20

1x 8 ml

Lyophilized Urine Calibrator Level 1

ZV-3032-02K1-20

1x 8 ml

Lyophilized Urine Control Level 1

ZV-3032-02K2-20

1x 8 ml

Lyophilized Urine Control Level 2

ZV-3032-02C1-20

1 x 1 pcs

Zivak Metanephrine Urine LC-MS/MS Analytical Column

 
File Name Type Size Download
User Manual 832.01 KB

Zivak General Catalog

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