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VMA, HVA, 5-HIAA LC-MS/MS analysis kit

VMA, HVA, 5-HIAA LC-MS/MS analysis kit

CE-IVD certified and suitable for any kind of LC-MS/MS instrument

 

The Zivak VMA, HVA, 5-HIAA LC-MS/MS analysis kit can be used in combination with the Zivak-Multitasker as well as manually. Analysis run time for the manual version is just 15 minutes. Due to the efficient and quite smart procedure of the Zivak-Multitasker, the automated version guarantees also to receive results in 15 minutes per test, including sample preparation-, sample injection- and analysis run time.

 
Quantitative LC-MS/MS analysis kit for VMA-HVA-5-HIAA in urine.

Vanillylmandelic acid (VMA) and homovanillic acid (HVA) are final products of the catecholamine metabolism in human body. Urinary concentrations of VMA and HVA are used for screening of neuroblastoma, one of the most important tumors in children. 5-hydroxyindoleacetic acid (5-HIAA) is a metabolite of serotonin and is used for the diagnosis of carcinoid tumors.

Zivak Urinary VMA, HVA, 5-HIAA LC-MS/MS Analysis Kit was developed for accurate analysis of VMA, HVA and 5-HIAA in urine samples.

Main methods and procedures that is selected by Zivak are based on EN ISO 13485 and 98/79/EC.

VMA, HVA, 5-HIAA LC-MS/MS analysis kit 0
LC-MS/MS Parameters

Device

Zivak Tandem Gold LC-MS/MS System

Column

Zivak VMA HVA 5-HIAA Urine LC-MS/MS Analytical Column

Injection Volume

20 µL

Pump Program

00:00  min                   90 % A

01:30  min                   90 % A

05:00  min                   70 % A

05.01  min                   60 % A

08:00  min                   60 % A

08:01  min                   40 % A

11.00  min                   40 % A

11.01  min                   90 % A

15:00  min                   90 % A

Flow

0.35 mL/min

Ionization Mode:

ESI Negative

CID Gas

2.4 mTorr

API Nebulizing Gas

50 psi

Scan Time

1.0 sec

SIM Width

1.5 amu

Drying Gas

35 psi

Drying Gas Temp.

350 °C

Needle

6000V

Shield

600V

Capillary

80V

Detector

1700 V

 
 
Analytical Performance

No

Analyte

LOD (µmol/L)

LOQ

(µmol/L)

Accuracy

(%)

Intra-Assay

Precision

 (%CV)

Inter-Assay

Precision

 (%CV)

Linearity

(R2)

1

VMA

0.3

0.9

97.5

2.9

4.3

0.9990

2

HVA

0.2

0.6

98.7

2.3

3.5

0.9992

3

5-HIAA

0.2

0.6

96.3

3.3

4.9

0.9989

 

Storage & Stability:

  • This analysis kit can be shipped at room temperature.
  • Urine calibrator and controls should be stored at between 2-8 °C.
  • After arrival, reagent 1 and urine calibrator and controls (after reconstitution) must be stored at -20 °C.
  • Reagent 2 must be stored at room temperature.
  • All other components of the kit can be stored at room temperature
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.  

 

Preparation of Calibrators:

  1. Add 5 ml of 0.2 M HCl to urine calibrator and dissolve.
  2. After preparation, calibrators must be aliquoted into 350 µl volumes and stored at -20 °C.

 

Preparation of Controls:

  1. Add 8 ml of 0.2 M HCl to urine control and dissolve.

  2. After preparation, controls must be aliquoted into 350 µl volumes and stored at -20 °C.

Sample Pretreatment (Manual):

  1. Take 300 mL of patient sample (or calibrators and controls) into a 1.5 mL capped preparation tube.
  2. Add 150 mL of Reagent 1 and vortex for 10 seconds.
  3. Add 60 mL of Reagent 2 and vortex for 10 seconds.
  4. Filter liquid with injector and 0.45 um filter.
  5. Take filtered sample into the autosampler vial.
  6. Inject 20 mL to the LC-MS/MS system.

Note: The prepared sample is stable at 2-4 °C for 24 hours.

 

Materials Supplied

Order No.

Volume

Component

ZV-3030-02R1-20

1x 40 ml

Reagent 1, Contains deuterated internal standards

ZV-3030-02R2-20

1 x 20 ml

Reagent 2, Contains acid

ZV-3030-02MA-20

2 x 1 L

Mobile Phase A, Contains organic solvent

ZV-3030-02MB-20

1 x 1L

Mobile Phase B, Contains organic solvent

   ZV-3030-02WB-10

1 x 1 L

Washing solution, Contains organic solvent

ZV-3030-KK-20

1 x 1 pc

User Guide

 
 

Materials Required But Not Supplied

Order No.

Volume

Component

ZV-3030-02S1-20

1 x 5 ml

Lyophilised Urine Calibrator Level 1

ZV-3030-02K1-20

1 x 8 ml

Lyophilised Urine Control Level 1

ZV-3030-02K2-20

1 x 8 ml

Lyophilised Urine Control Level 2

ZV-3030-02C1-20

1 pcs

Zivak VMA HVA 5-HIAA Urine LC-MS/MS Analytical Column

 
File Name Type Size Download
User Manual 829.56 KB

Zivak General Catalog

Download Zivak General Catalog PDF