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DOA (Drug of Abuse) LC-MS/MS analysis kit

DOA (Drug of Abuse) LC-MS/MS analysis kit
CE-IVD certified and suitable for any kind of LC-MS/MS instrument
 

Zivak LC-MS/MS Psychoactive drugs analysis kit can be used in combination with the Zivak-Multitasker as well as manually. 

Zivak Psychoactive Drugs LC-MS/MS Analysis Kit is developed for quantitative detection of 15 selected psychoactive drugs from different drug groups in urine samples with a minimum sample preparation in 7 minutes. Main methods and procedures that have been selected are based on EN ISO 13485 and 98/79/EC.

 

Quantitative LC-MS/MS analysis kit for Psychoactive Drugs in urine.

Psychoactive drugs are chemical substances that affect the brain functions, behaviours and consciousness. These drugs can be used therapeutically to treat both physical and psychological disorders. Monitoring the concentrations of these drugs is very important issue for clinicians if they used for therapy. The analytic methods must be highly sensitive and selective for accurate and precise quantification of these drugs for therapeutic monitoring. Detection and quantification of these drugs can be performed using UV or fluorescence spectrophotometers, immunoassays and radio-receptor assays. Mass spectrometers coupled with chromatographic systems such as LC-MS, LC-MS/MS, GC-MS are more suitable for this purpose through their sensitivity and specifity.

Zivak Psychoactive Drugs LC-MS/MS Analysis Kit is developed for quantitative detection of 15 selected psychoactive drugs from different drug groups in urine samples with a minimum sample preparation in 7 minutes. Main methods and procedures that have been selected are based on EN ISO 13485 and 98/79/EC.

DOA (Drug of Abuse) LC-MS/MS analysis kit 0 DOA (Drug of Abuse) LC-MS/MS analysis kit 1 DOA (Drug of Abuse) LC-MS/MS analysis kit 2 DOA (Drug of Abuse) LC-MS/MS analysis kit 3 DOA (Drug of Abuse) LC-MS/MS analysis kit 4
LC-MS/MS Parameters

Device

Zivak Tandem Gold LC-MS/MS System

Column

Zivak Psychoactive Drugs Urine LC-MS/MS Analytical Column

Injection Volume

20 µL

Pump Program

00:00  min                   5          %B

01:00  min                   5          %B

03:00  min                   95         %B

04:30  min                   95         %B

04:31  min                   5          %B

07:00  min                   5          %B

Flow

0.25 mL/min

Ionization Mode

vESI positive

CID Gas

2.4mTorr

API Nebulizing Gas

55 psi

Scan Time

0,725 sec

SIM Width

1.0 amu

Drying Gas

35 psi

Drying Gas Temp.

350 °C

Vortex Gas

25psi

Vortex Gas Temp.

250°C

Needle

5000V

Shield

600V

Capillary

50V

Detector

1600 V

 
 
Analytical Performance

No

Analyte

   Linearity

  (R2 ; 0-100µg/L)

  Recovery

        (%) 

    Limit of          Detection       (µg/L)

     Limit of        Quantitation

  (µg/L)

   Intra-Assay

     Precision

      (%CV)

   Inter-Assay

    Precision

      (%CV)

1

Amphetamine 

   0.9994

    99.24

0.27

0.90

2.6

2.9

2

Methamphetamine

0.9998

100.88

0.31

1.02

4.8

5.3

3

MDA

0.9976

95.32

0.08

0.26

2.6

2.9

4

MDMA

0.9991

98.54

0.12

0.39

2.9

3.3

5

MDEA

0.9998

98.74

0.07

0.23

3.4

3.7

6

Diazepam

0.9999

95.86

0.10

0.33

2.4

2.6

7

Morphine

0.9999

100.48

0.08

0.26

6.7

6.9

8

Codeine

0.9969

92.10

0.31

1.02

4.4

4.7

9

Cocaine

0.9989

96.84

0.09

0.30

2.8

3.1

10

Alprazolam

0.9996

96.96

0.09

0.30

2.8

3.2

11

Delta-9-THC

0.9998

98.82

0.50

1.65

4.1

4.5

12

Clonazepam

0.9998

97.42

0.16

0.53

2.9

3.3

13

Flunitrazepam

0.9996

98.76

0.13

0.43

4.4

4.7

14

6-acetylmorphine

0.9986

99.12

0.08

0.26

2.6

2.8

15

Heroin

0.9987

98.10

0.12

0.40

6.3

6.7

 

Storage & Stability

  • Please check the labels on reagent bottles. Reagents of this kit contain hazardous material may cause eye and skin irritations. This analysis kit can be shipped at room temperature.
  • After arrival, reagent 2 and standard 1 must be stored at -20 °C.
  • Synthetic Urine must be stored at 2-8 °C
  • All other components must be stored at room temperature. Please make sure that you keep reagent 1 at room temperature otherwise it will become unusable.
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

Preparation of Zivak Calibrator Level I:

Add 1900 µl synthetic urine into a preparation tube.

Add 100 mL of Standard and vortex for 10 seconds.

After preparation, calibrator must be aliquoted into 350 µl volumes and stored at -20°C.

 
 

Preparation of Zivak Calibrator Level II:

Add 1800 µl synthetic urine into a preparation tube.

Add 200 mL of Standard and vortex for 10 seconds.

After preparation, calibrator must be aliquoted into 350 µl volumes and stored at -20°C.

 
 

Preparation of Zivak Control Level I:

Add 1850 µl synthetic urine into a preparation tube.

Add 150 mL of Standard and vortex for 10 seconds.

After preparation, control must be aliquoted into 350 µl volumes and stored at -20°C.

 
 

Sample Pre-treatment (Manual)

Take 300 mL of urine sample (or calibrator) into a preparation tube (centrifuge first if the sample is blurry)

 

Add 250 mL of Reagent 1 and vortex for 10 seconds.

 

Add 300 mL of Reagent 2 and vortex for 10 seconds.

 

Centrifuge at 5.000 x rpm for 3 minutes.

 

Take 200mL of upper phase into the vials.

 

Inject 20 mL to the LC-MS/MS system.

 

Note: The prepared sample is stable at 2-4 °C for 24 hours.

 

Sample Pre-treatment (Automated):

Take 300 mL of patient sample (or calibrators and controls) into the Zivak Multitasker Sample Preparation vials

 

Make sure that reagents and wash solution are connected to the reagent pump and purged properly. 

 

Place the vials on the MULTITASKER system tray.

 

Note: The prepared sample is stable at 2-8°C for 24 hours.

Materials Supplied

Order No

Volume

Content

ZV-2001-02R1-20

2 x 37 ml

Reagent 1, Contains base

ZV-2001-02R2-20

 2x 45 ml

Reagent 2, Contains internal standards

ZV-2001-02MA-20

 1x 0,5 L

Mobile Phase A, Contains organic solvent and acid

ZV-2001-02MB-20

 1x 0,5 L

Mobile Phase B, Contains organic solvent and acid

ZV-2001-02WB-10

 2 x 1 L

Washing solution, Contains organic solvent

ZV-2001-02SU-10

 1x 10ml

Synthetic Urine

ZV-2001-KK-20

    1x 1 pc

User Guide

 
 
Materials Required But Not Supplied

Order No

Volume

Content

ZV-2001-02S1-20

1 x 1 ml

Standard 1, in organic solvent

ZV-2001-02C1-20

1 pcs

Zivak Psychoactive Drugs Urine LC-MSMS Analytical Column

 
File Name Type Size Download
User Manual 1.15 MB

Zivak General Catalog

Download Zivak General Catalog PDF