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Haemoglobin A1c HPLC-UV Analysis Kit

 Haemoglobin A1c HPLC-UV Analysis Kit
Zivak Hemoglobin A1c HPLC Analysis Kit was developed for screening and monitoring diabetes mellitus in human blood samples. The analysis time is 1.6 minutes. Main methods and procedures that we selected are based on EN ISO 13485 and 98/79/EC.

The amount of HbA1c in whole blood is one of the most important parameters for diabetes screening and monitoring. HbA1c is formed by combination of hemoglobin protein and sugar in the blood. The production of HbA1c rises when the rate of blood sugar increases. Therefore, the amount of HbA1c is directly related with high blood glucose. The different types of HbA1c molecules can be occurred such as A1a, A1b, s-A1c, L-A1c, according to the type of sugar which combined to the hemoglobin. The most important part of the HbA1c analysis is the best separation of these species.

 Haemoglobin A1c HPLC-UV Analysis Kit 0
HPLC-UV Parameters

Instrument

Zivak HB-100 Full Automated HbA1c/Variant HPLC Analyzer

Column

Zivak Hemoglobin A1c HPLC Column

Detector

UV/VIS

Wavelength

415 nm

Injection Volume

20 µL

Column Oven Temperature

35 °C

Analytical Performance

No

Analyte

Accuracy

(%)

Intra-assay Precision

(%CV)

Inter-assay Precision

(%CV)

Linearity

(R2)

1

HbA1c

98.32

0.479

1.19

0.9985

 

 

 

 

 

 

Storage & Stability:

  • This analysis kit can be shipped at room temperature.
  • After arrival, mobile phases and reagent 1 must be stored at room temperature.
  • Whole blood controls and column must be stored at 2-8 °C until reconstitution.
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

Preparation of Calibrators & Controls:

  • Calibrators & controls are ready to use. No sample pretreatment needed.
  • Calibrators & controls must be stored at +4 °C temperature.

 

Sample Pretreatment (Manual):

  Take 5 mL patient sample into a glass vial.

 

Add 1 mL of Reagent 1 and vortex for 10 seconds.

 

Inject 20 mL to the HPLC-UV system.

 

Prepared samples are stable for 24 hours at 2-4 °C temperature.

 

Sample Pretreatment (Automated):

Place whole blood EDTA samples into system tray.

 

Analysis will automatically start after clicking Start button on software.

                                                                  

Injections, calculations and reporting done automatically by instrument.

 

Prepared samples are stable for 24 hours at 2-4 °C temperature.

 

Materials Supplied

Order No

Volume

Component

ZV-4001-05R1-25

4 x 2 L

Reagent 1, Contains aqueous buffer

ZV-4001-05MA-25

1 x 2.1 L

Mobile Phase A, Contains organic solvent

ZV-4001-05MB-25

1 x 0.9 L

Mobile Phase B, Contains organic solvent

ZV-4001-KK-25

1 x 1 pc

User Guide

 
 
Materials Required But Not Supplied

Order No

Volume

Component

ZV-4001-02S1-25

1 x 3 mL

Hemolysate Calibrator Level 1

ZV-4001-02S2-25

1 x 3 mL

Hemolysate Calibrator Level 2

ZV-4001-02K1-25

1 x 3mL

Hemolysate Control Level 1

ZV-4001-02K2-25

1 x 3 mL

Hemolysate Control Level 2

ZV-4001-02C1-25

1 x 1 pc

Zivak Hemoglobin A1c Whole Blood HPLC Analytical Column

 
File Name Type Size Download
User Manual 935.2 KB

Zivak General Catalog

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