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Hemoglobin Variants HPLC-UV Analysis Kit

 Hemoglobin Variants HPLC-UV Analysis Kit

Zivak Hemoglobin Variants HPLC Analysis Kit was developed for accurate analysis of diseases related with Hb variants in human blood samples. The analysis time is 6.5 minutes. Main methods and procedures that have been selected are based on EN ISO 13485 and 98/79/EC.

Hemoglobin variants are abnormal forms of hemoglobin. Normal hemoglobin types in human blood include:

-           HbA makes up about 95% - 98% of Hb found in adults

-           HbA2 makes up about 2% - 3% of Hb found in adults

-           HbF makes up about 2% of Hb found in adults

Hemoglobin variants occur when genetic changes in the globin genes cause alterations in the amino acids that make up the globin protein. These changes may affect the structure of the hemoglobin. There are four genes that code for alpha globin chains and two genes that code for the beta globin chains. The most common alpha-chain-related condition is alpha thalassemia and most common beta-chain-related condition is beta thalassemia.

In thalassemia diseases, mutations of one or more globin genes cause reduction of globin chain production. As a result of this situation the percentage of minor hemoglobins (HbA2 and HbF) increases.

Hemoglobin E is one of the most common beta chain hemoglobin variants. People who are homozygous for HbE generally have a mild hemolytic anemia and a mild enlargement of the spleen.

HbC disease is rare and relatively mild. It usually causes a minor amount of hemolytic anemia and a mild to moderate enlargement of the spleen.

HbS is the primary hemoglobin in people with sickle cell disease.

 Hemoglobin Variants HPLC-UV Analysis Kit 0
HB-100 LC parameteres : contact for more details

Storage & Stability: 

  • This analysis kit can be shipped at room temperature.
  • After arrival, mobile phases and reagent 1 must be stored at room temperature.
  • Whole blood calibrators and controls must be stored at 2-8 °C until reconstitution.
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

Preparation of Sample , Calibrators & Controls: contact for more details

 

Materials Supplied

Order No

Volume

Component

ZV-4002-05R1-25

5 x 2 L

Reagent 1, Contains aqueous buffer

ZV-4002-05MA-25

3 x 1.7 L

Mobile Phase A, Contains organic solvent

ZV-4002-05MB-25

1 x 2,1 L

Mobile Phase B, Contains organic solvent

ZV-4002-KK-25

1 x 1 pc

User Guide

 
 
Materials Required But Not Supplied

Order No

Volume

Component

ZV-4002-02S1-25

1 x 3 mL

Whole Blood Calibrator Level 1

ZV-4002-02S2-25

1 x 3 mL

Whole Blood Calibrator Level 2

ZV-4002-02K1-25

1 x 3 mL

Whole Blood Control Level 1

ZV-4002-02K2-25

1 x 3 mL

Whole Blood Control Level 2

ZV-4002-02C1-25

1 x 1 pc

Zivak Hemoglobin Variant Whole Blood HPLC Analytical Column

 

Zivak General Catalog

Download Zivak General Catalog PDF