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Carnitine / Acylcarnitines LC-MS/MS Analysis Kit (Dried Blood Spot)

Carnitine / Acylcarnitines LC-MS/MS Analysis Kit	(Dried Blood Spot)

Carnitine / Acylcarnitines LC-MS/MS analysis kit for dried blood spot.

Carnitine / Acylcarnitines screening is the most important part of the clinical diagnosis of amino acid, fatty acid or organic acid metabolism. Carnitine / Acylcarnitines screening allows to the diagnosis of over 30 metabolic diseases from a drop of blood samples taken with a screening card. This screening test occurred by measurement of Carnitine / Acylcarnitins with isotope dilution tandem mass spectrometry. LC-MS/MS is a powerful tool for gives results Carnitine / Acylcarnitines in 1.5 minutes.

Screening of following analytes can be performed by Zivak Carnitine / Acylcarnitine LC- MS/MS Analysis Kit and Zivak NeoZin Screening software. Main methods and procedures that have been selected are based on EN ISO 14971.

By the quantitative analysis of carnitine esters including such as free carnitine, C0, C2, C4, C6, C8, C10, C12, C14, C16, C16 OH, C18 , C18 OH carnitine disorders which are related with fatty acid oxidation.

By the quantitative analysis of carnitine esters including such as C3, C3DC, C4OH, C5, C5OH,C5DC carnitine, disorders which are related with organic acid metabolism.

Carnitine / Acylcarnitines LC-MS/MS Analysis Kit	(Dried Blood Spot) 0 Carnitine / Acylcarnitines LC-MS/MS Analysis Kit	(Dried Blood Spot) 1
LC-MS/MS Parameters

Device

LC-MS/MS Analysis System

Column

Zivak Carnitine / Acylcarnitines Screening Dried Blood Spots LC-MS/MS Trap Column Housing - Cartridge

Injection Volume

20 µL

Pump Program

00:00  min                   100% B

01:30  min                   100% B

Flow

0.30 mL/min

Ionization Mode:

ESI positive

CID Gas

2.0 mTorr

API Nebulizing Gas

55 psi

Scan Time

1.519 sec

SIM Width

1.5 amu

Drying Gas

30 psi

Drying Gas Temp.

350 °C

Needle

5000V

Shield

600V

Capillary

30V

Detector

1500 V

 

 

Analytical Performance

No

Analyte

LOD (µmol/L)

LOQ (µmol/L)

Accuracy

(%)

Intra-Assay

Precision  (%CV)

Inter-Assay

Precision

(%CV)

Linearity

(R2)

1

C0 Carnitine

0,023

0,070

98,7

4,02

0,14

0,9983

2

C2 Carnitine

0,042

0,126

99,7

3,21

0,04

0,9984

3

C3 Carnitine

0,024

0,073

99,7

2,30

0,01

0,9994

4

C4 Carnitine

0,054

0,163

98,8

4,44

0,12

0,9993

5

C5 Carnitine

0,023

0,070

99,0

3,44

0,00

0,9937

6

C4OH Carnitine

0,047

0,142

95,0

3,12

0,08

0,9997

7

C6 Carnitine

0,041

0,122

100,6

2,50

0,14

0,9978

8

C5OH Carnitine

0,022

0,066

102,4

3,40

0,15

0,9957

9

C8 Carnitine

0,088

0,263

99,7

3,41

0,06

0,9943

10

C3DC Carnitine

0,068

0,205

104,6

3,66

0,23

0,9989

11

C10 Carnitine

0,088

0,265

105,3

4,95

0,21

0,9962

12

C5DC Carnitine

0,076

0,227

101,7

2,87

0,15

0,9969

13

C12 Carnitine

0,068

0,205

104,7

4,08

0,52

0,9984

14

C16OH Carnitine

0,090

0,269

96,7

4,45

0,14

0,9967

15

C14 Carnitine

0,067

0,200

95,3

3,78

0,08

0,9945

16

C16 Carnitine

0,077

0,232

100,2

1,39

0,02

0,9943

17

C18OH Carnitine

0,091

0,273

95,0

3,84

0,19

0,9936

18

C18 Carnitine

0,042

0,127

100,4

3,67

0,37

0,9948

 

 

Storage & Stability

  • It’s recommended to work in a fume hood for your safety as Reagent 2 is volatile and please avoid inhaling.
  • This analysis kit can be shipped at room temperature.
  • Controls, Reagent 2, 3 should be stored at 2-8 °C.
  • Reagent 1 should be stored at -20 °C.
  • All other components of the kit can be stored at room temperature.
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

Sample Pre-treatment (Manual)

Take dried blood spot sample in 3.5 mm diameter by a puncher on a 96-well microplate.

 

Add 200 mL of R1 to each well. Incubate for 30 minutes at room temperature.

 

After incubation, take the liquid phase on another well. Then dry with warm air flow.

 

Add 60 mL of R2 on dried samples.

 

Cover microplate properly with alumina foil or appropriate seal And incubate for 20 minutes at 65 °C.

 

Remove alumina foil and dry the liquid phase completely with warm air flow.

 

Add 200 µl of R3 to dissolve the dried wells.

                                                                                     

Take the liquid into a vial or directly inject 20 µl from 96 well plate to LC-MS/MS system.

 

Note: The prepared sample is stable at 2-8°C for 24 hours.

Materials Supplied

Order No.

Volume

Component

ZV-3051-02R1-15

1 x 40 ml

Reagent 1, Contains deuterated internal standards

ZV-3051-02R2-15

1 x 12 ml

Reagent 2, Contains derivatization reagent

ZV-3051-02R3-15

1 x 40 ml

Reagent 3, Contains acid

  ZV-3051-02WB-15

 1 x 0,5 L

Washing solution, Contains organic solvent

ZV-3051-02MP-15

1 x  0,6 L

Mobile Phase, Contains organic solvent

ZV-3051-KK-15

1 x 1 pc

User Manual

 
Materials Required But Not Supplied

Order No.

Volume

Component

ZV-3051-02K1-15

1 x 1 Spot

Dried Blood Spot Control Level 1

ZV-3051-02K2-15

1 x 1 Spot

Dried Blood Spot Control Level 2

ZV-3051-02S1-15

1 x 1 Spot

Dried Blood Spot Calibrator Level 1

ZV-3051-02S2-15

1 x 1 Spot

Dried Blood Spot Calibrator Level 2

ZV-3051-02C1-15

1 x 1 pcs

Zivak Carnitine / Acylcarnitines Dried Blood Spots LC-MS/MS Trap Column Holder

ZV-3051-CSPE-15

1 x 1 pcs

Carnitine / Acylcarnitines Dried Blood Spots LC-MS/MS Trap Column Cartridge

 

 

 

 

 

 

 

 

 

 

 

 

 

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User Manual 894.53 KB

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