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73 Organic Acids LC-MS/MS analysis kit

73 Organic Acids LC-MS/MS analysis kit
CE-IVD certified and suitable for any kind of LC-MS/MS instrument

 

The Zivak 73 Organic Acids LC-MS/MS analysis kit can be used in combination with the Zivak-Multitasker as well as manually. Analysis run time for the manual version is 20 minutes. Due to the efficient and quite smart procedure of the Zivak-Multitasker, the automated version guarantees to receive quantitative results in just 20 minutes per test, including sample preparation-, sample injection- and analysis run time. Additionally, high accuracy is being rectified through external quality tests.

 

Quantitative LC-MS/MS analysis kit for Organic Acids in human urine samples.

Organic acid disorders (organic acidemia) are a group of inherited metabolic conditions. Most of the organic acidemias caused by defective autosomal genes for various enzymes for amino acid metabolism. Neurological and physiological harms are caused by this impaired ability to synthesize a key enzyme required to break down a specific amino acid, or a group of amino acids, resulting in acidemia and toxicity to specific organs systems. Most are inherited as autosomal recessive diseases. Organic acid related disorders may affect many metabolic pathways including amino acid, lipid metabolism, fatty acid oxidation and the Krebs cycle.

Organic acidemias are usually diagnosed in infancy, characterized by urinary excretion of abnormal amounts or types of organic acids. The diagnosis is usually made by detecting an abnormal pattern of organic acids in a urine sample. In some conditions, the urine is always abnormal, in the others the characteristic substances are only present intermittently. Many of the organic acidemias are detectable by newborn screening with Tandem Mass spectrometry. These disorders vary in their prognosis, from manageable to fatal, and usually affect more than one organ system, especially the central nervous system.

Quantitative analysis of the organic acids in urine samples have clinical importance for determining organic acidemias and monitoring the organic acid concentrations in human urine. ZIVAK Quantitative Organic Acid LC-MS/MS kit was developed for quantitative detection of the 73 major organic acids in human urine samples. LC-MS/MS is a influential device for the study of metabolic disorders and LC-MS/MS analysis gives results in 20 minutes with minimal sample preparation. Main methods and procedures that we selected are based on EN ISO 13485 and 98/79/EC.

 

73 Organic Acids LC-MS/MS analysis kit 0 73 Organic Acids LC-MS/MS analysis kit 1
LC-MS/MS Parameters

Device

Zivak Tandem Gold LC-MS/MS System

Column

Organic Acid Urine LC-MS/MS Analytical Column

Injection Volume

20 µL

Pump Program

00:00  min                                         0     % B

14:00  min                                         100 % B

16:00  min                                         100 % B

16:01  min                                         0     % B

20:00  min                                         0     % B

Flow

0.25 mL/min

Ionization Mode

ESI positive + ESI negative

CID Gas

2.4 mTorr

API Nebulizing Gas

55 psi

Scan Time

1,13 sec

SIM Width

1,2 amu

Drying Gas

35 psi

Drying Gas Temp.

350 °C

Needle

5000V

Shield

600V

Capillary

40V

Detector

1600 V

 
 
Analytical Performance

 

 

 

 

 

Inter-Assay Precision (%CV)

 

No

Analyte

LOD (mg/L)

LOQ (mg/L)

Linearity (r2)

1.Day %RSD

2.Day %RSD

3.Day %RSD

% Recovery

1

2,3-pyridinedicarboxylic acid

0,0070

0,0240

0,9939

6,53

2,45

4,08

105,0

2

2-Hydroxyphenylacetic acid (o-Hydroxyphenylacetic acid)

0,0350

0,1150

0,9984

5,97

6,90

3,60

94,4

3

2-Ketoglutaric acid (Alpha-ketoglutaric acid)

0,0300

0,1000

0,9996

5,88

6,08

5,56

96,0

4

2-Methylcitric acid

0,0170

0,0570

0,9966

6,22

6,58

5,83

95,6

5

2-Methylglutaric acid (Methylglutaric acid)

0,0040

0,0130

0,9995

5,62

7,02

2,94

93,3

6

2-Methylhippuric acid

0,0010

0,0030

0,9994

2,45

5,12

1,43

100,1

7

3,4-Dihydroxyhydrocinnamic acid

0,0100

0,0330

0,9983

3,30

1,57

2,14

94,8

8

3-Hydroxy-3-methylglutaric acid

0,0070

0,0220

0,9980

3,62

3,55

5,48

99,6

9

3-Hydroxybutyric acid (β-Hydroxybutyric acid)

0,0020

0,0060

0,9995

6,11

5,10

5,66

96,4

10

3-Hydroxypropanoic acid

0,0100

0,0330

0,9963

2,57

5,25

5,04

93,8

11

3-İndoleacetic acid

0,0000

0,0010

0,9982

2,82

8,74

6,85

94,0

12

3-methyl-2-oxobutanoic acid sodium salt (alpha-Ketoisovaleric acid)

0,0020

0,0060

0,9952

2,33

2,84

1,25

99,7

13

3-Methyl-2-oxovaleric acid

0,0260

0,0850

0,9964

4,01

1,47

2,56

92,5

14

3-Methylcrotonyl Gylcine

0,0100

0,0340

0,9950

6,34

9,95

7,01

100,2

15

3-Methylglutaric acid

0,0080

0,0260

0,9996

7,04

7,01

6,01

93,3

16

3-methylhippuric acid

0,0010

0,0020

0,9984

6,27

4,04

8,25

104,1

17

3-Phenyllactic acid

0,0030

0,0090

0,9996

3,05

9,73

7,07

96,1

18

4,6-Dioxoheptanoic acid (Succinylacetone)

0,0120

0,0400

0,9948

3,01

2,55

6,35

98,6

19

4-Hydroxy-3-methoxymandelic Acid (Vanilmandellic Acid)

0,0000

0,0010

0,9968

8,21

7,56

2,97

103,0

20

4-Hydroxybenzoic acid

0,0040

0,0140

0,9993

2,08

4,31

4,23

90,4

21

4-Hydroxyphenylacetic acid (p-Hydroxyphenylacetic acid)

0,0020

0,0050

0,9992

4,60

5,43

8,66

93,2

22

4-Methylhippuric acid

0,0010

0,0020

0,9909

8,00

5,63

7,33

98,1

23

5-Hydroxyindole-3-acetic acid

0,0010

0,0020

0,9988

4,59

5,55

5,46

93,2

24

Adipic acid

0,0120

0,0410

0,9996

6,97

4,52

8,90

101,2

25

Alpha-Ketobutyric acid

0,0020

0,0070

0,9913

2,28

2,15

2,36

104,7

26

Alpha-Ketoisocaproic acid (4-Methyl-2-oxovaleric acid)

0,0040

0,0140

0,9992

2,69

2,58

4,12

101,1

27

Alpha-OH-butyric acid

0,0120

0,0400

0,9953

2,36

6,25

2,36

89,6

28

α-Hydroxyglutaric acid

0,0050

0,0160

0,9992

7,64

3,74

7,44

96,6

29

α-Hydroxyisovaleric acid

0,0050

0,0170

0,9977

6,14

8,81

9,42

97,5

30

β-Hydroxyisovaleric acid

0,0010

0,0040

0,9981

6,18

8,45

8,51

99,6

31

Benzoic acid

0,0120

0,0400

0,9995

2,30

7,84

5,16

97,6

32

Cis-aconitic

0,0030

0,0100

0,9948

3,47

4,39

5,48

101,2

33

Citramalic

0,0020

0,0070

0,9910

4,18

3,26

3,47

87,8

34

Citric acid

0,0810

0,2690

0,9973

3,20

1,68

1,31

101,1

35

D-Saccharic acid potassium salt (Glucaric Acid)

0,0010

0,0040

0,9952

8,97

8,91

6,77

98,5

36

Ethylmalonic acid

0,0040

0,0130

0,9985

3,83

2,17

8,19

93,8

37

Formiminoglutamic

0,0040

0,0140

0,9914

1,88

4,66

4,01

101,4

38

Fumaric acid

0,0380

0,1260

0,9984

3,59

5,79

1,50

99,3

39

Glutaric acid

0,0000

0,0010

0,9899

5,50

7,25

6,61

95,0

40

Glyceric acid hemicalciyum salt monohydrate

0,0100

0,0330

0,9960

2,04

4,54

6,62

93,1

41

Glycolic acid

0,0050

0,0170

0,9961

1,95

3,56

2,54

94,3

42

Hexanoyl glycine

0,0000

0,0020

0,9899

5,27

7,40

6,46

91,2

43

Hippuric acid

0,0260

0,0850

0,9988

2,26

6,68

2,58

95,4

44

Homovanillic acid

0,0030

0,0100

0,9901

2,54

3,65

4,25

94,2

45

Isocitric acid

0,0360

0,1190

0,9982

3,08

6,38

6,87

93,6

46

Kynurenic acid

0,0020

0,0070

0,9976

4,47

7,36

8,60

90,5

47

Lactic acid

0,0650

0,2150

0,9994

8,87

5,88

7,48

105,8

48

Malic acid

0,0020

0,0060

0,9991

8,50

7,77

7,19

99,3

49

Malonic acid

0,0080

0,0260

0,9982

5,49

8,95

6,72

95,9

50

Mandelic acid

0,0690

0,2270

0,9963

3,02

8,46

9,06

100,1

51

Methylmalonic acid

0,0110

0,0370

0,9938

5,06

5,99

4,67

105,2

52

Methylsuccinic acid (2-Methylsuccinic Acid)

0,0020

0,0060

0,9998

3,67

4,52

7,49

87,5

53

Mevalonolactone

0,0010

0,0050

0,9933

7,53

7,88

10,23

89,7

54

N-(3-Phenylpropionyl)glycine

0,0010

0,0040

0,9939

4,52

6,01

7,29

85,4

55

N-Acetyl-L-aspartic acid

0,0050

0,0170

0,9987

2,98

4,23

4,31

96,6

56

N-Isovalerylglycine

0,0010

0,0040

0,9988

5,19

3,19

5,99

89,2

57

Orotic acid

0,0190

0,0630

0,9979

7,68

8,79

8,31

103,1

58

Phenylacetic

0,0050

0,0170

0,9960

1,25

1,36

1,67

85,6

59

Phenylglyoxylic acid (Benzoylformic acid) (Benzoylform)

0,0030

0,0100

0,9887

7,15

7,01

9,05

102,9

60

P-Hydroxyphenyllactic acid

0,0040

0,0120

0,9977

5,97

3,39

5,16

98,9

61

Picolinic acid

0,0010

0,0030

0,9927

2,32

5,36

3,99

90,3

62

Pimelic acid

0,0010

0,0030

0,9987

4,69

4,07

8,71

81,7

63

Propionyl Glycine (2-propanamidoacetic acid)

0,0050

0,0170

0,9878

3,44

7,61

4,61

90,8

64

Pyroglutamic

0,0020

0,0060

0,9967

2,68

2,36

2,54

93,6

65

Pyruvic acid

0,0480

0,1580

0,9961

2,30

3,85

8,13

94,8

66

Sebacic acid

0,0000

0,0010

0,9956

3,10

6,34

9,52

106,2

67

Suberic acid

0,0020

0,0070

0,9981

5,13

7,03

6,66

99,6

68

Suberyl glycine

0,0010

0,0020

0,9936

4,66

8,85

6,44

87,1

69

Succinic acid

0,0720

0,2370

0,9968

7,95

4,46

3,36

105,4

70

Tartaric acid

0,0030

0,0100

0,9991

4,88

3,76

6,49

97,8

71

Tiglyl glycine

0,0000

0,0010

0,9982

4,42

3,65

4,72

100,8

72

Tricarballylic acid

0,0010

0,0020

0,9990

4,91

4,95

2,80

83,0

73

4,8-Dihydroxyquinoline-2-carboxylic acid (Xanthurenic acid)

0,0020

0,0060

0,9992

6,89

4,42

6,64

83,7

 

Storage & Stability:

  • This analysis kit can be shipped at room temperature. 
  • After arrival, reagent 1, 2, 3, calibrators and controls should be stored at 2-8 °C.  
  • All other components of the kit should be stored at room temperature.
  • All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions.

Preparation of Calibrators & Controls:

Add 3 ml deionized water to urine Calibrator/Control and dissolve.

After preparation, calibrators & controls must be aliquoted into 350 µl volumes and stored at -20°C.

 
Sample Pretreatment (Automated):
 

Take 300 mL of patient sample (or calibrators and controls) into the Zivak Multitasker Sample Preparation vials

 

Make sure that reagents and wash solution are connected to the reagent pump and purged properly. 

 

Place the vials on the MULTITASKER system tray.

 

Note: The prepared sample is stable at 2-8°C for 24 hours.

 

Sample Pretreatment (Manual):

 

Take 300 mL of patient sample (or calibrators and controls) into the autosampler vial

 

Add 50 µL of Reagent 1 and vortex for 5 seconds.

 

Add 50 µL of Reagent 2-3 mixture (described above) and vortex for 5 seconds

 

Inject 20 mL to the LC-MS/MS system.

Note: The prepared sample is stable at 2-8°C for 24 hours.

Materials Supplied

Order No.

Volume

Component

ZV-3004-02R1-20

2 x 6 ml

Reagent 1, Contains internal standards

ZV-3004-02R2-20

1 x 6 ml

Reagent 2, Contains acid

ZV-3004-02R3-20

1 x 6 ml

Reagent 3, Contains stabilizer

ZV-3004-02MA-20

1 x 1 L

Mobile Phase A, Contains organic solvent

ZV-3004-02MB-20

1 x 0,5 L

Mobile Phase B, Contains organic solvent

ZV-3004-02WB-10

 1 x 1 L

Washing solution, Contains organic solvent

ZV-3004-KK-20

1 x 1 pc

User Guide

 
 
Materials Required But Not Supplied

Order No.

Volume

Component

ZV-3004-02S1-20

1 x 3 ml

Lyophilized Urine Calibrator Level 1

ZV-3004-02S2-20

1 x 3 ml

Lyophilized Urine Calibrator Level 2

ZV-3004-02S3-20

1 x 3 ml

Lyophilized Urine Calibrator Level 3

ZV-3004-02K1-20

1 x 3 ml

Lyophilized Urine Control Level 1

ZV-3004-02K2-20

1 x 3 ml

Lyophilized Urine Control Level 2

ZV-3004-02C1-20

1 pcs

Zivak Organic Acid Urine LC-MS/MS Analytical Column

   ZS-2ML-8-1001

2x100pcs

  2 ml glass autosampler vial

   ZS-9004-0100-00

2x100pcs

  2 ml glass autosampler vial caps pre-slit

 
File Name Type Size Download
User Manual 1.43 MB

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